| Congresso Brasileiro de Microbiologia 2023 | Resumo: 115-2 | ||||
Resumo:Color plays an important role in the food industry because the visual appearance of a product is directly related to its flavor and aroma promise. Colors can be obtained through natural or synthetic colorants. Synthetic colorants can be derived from petroleum and can cause adverse effects on human health and the environment. Thus, natural colorants, which can come from microorganisms, have emerged as an alternative. The objective of this research was to evaluate the effect of different types of culture media on the production of Natural Red Colorant (NRC) by Penicillium chrysogenum, both with and without shaking. The fungus used in the preset study was Penicillium chrysogenum, isolated from a compost bin. The reactivation of the fungus was conducted on potato dextrose agar (PDA) medium for 7 days at 28 °C. For the tests, four different types of liquid media were evaluated, named as medium BD (20 g.L -1 dextrose, and 200 g.L -1 potato broth), medium 1 (B-YE) (200 g.L -1 potato broth, 5 g yeast extract, distilled water q.s. 1 L), medium 2 (CG) (30 g glucose, 10 g meat extract, 10 g meat peptone, distilled water q.s. 1 L) and medium 3 (MSG-glucose) (10g glucose, 25g monosodium glutamate (MSG), 0.012 g MgSO 4 .H 2 0, distilled water q.s. 1L). Cultivation was performed at 200 rpm shaking and steady state conditions for 5 days to evaluate NRC production under stress. The assays were conducted in 125 mL Erlenmeyer flasks with 50 mL of culture medium (40 % of the total volume) after autoclave sterilization. The flasks were inoculated and stored at 30 °C. Initially, 1 mL of fungal spore suspension at a concentration of 108 mL-1 was used as inoculum. However, inoculation using fragments of the fungal growth from solid medium proved to be more suitable for dye excretion by the fungus. Therefore, the media were inoculated with three discs (11.1 mm in diameter, totaling an area of 290.31 mm2) of fungal mycelium as an inoculum. The quantification of the colorant was performed by spectrophotometer reading of the filtered liquid media on filter paper at wavelengths of 400 nm, 470 nm, and 490 nm. Initially the PD medium was tested to observe the potential production in a liquid medium at 30 °C without shaking. The effect of pH on excretion into the PD liquid medium was evaluated within the pH range of 4 to 9. It was observed that the highest production of biocolorants occurred at a basic pH, particularly at pH 8 and 9. When the fungus was inoculated on BD medium at pH 8 and 9, there was less dye excretion compared to the stationary condition. When testing the other culture media media, the fungus exhibited different behavior compared to the BD medium. Colorant production occurred at 200 rpm agitation for all culture media, with medium 3 standing out for NRC production. The results obtained in the present work demonstrate that the fungus C1I3 belonging to the species Penicillium chrysogenum has great potential for NRC production and it was evident that the production is highly dependent on nutritional and environmental conditions. Palavras-chave: colorant, culture medium, environmental conditions, fungi, human health Agęncia de fomento:UNESP | |||||